The Dutch meeting on Molecular and Cellular Biophysics was held on Monday 3 and Tuesday 4 October 2011. This year the meeting was held as a joint event with Annual Meeting of the Netherlands Society for Microscopy (NVvM). Next year the Dutch meeting on Molecular and Cellular Biophysics will be on Monday 1 and Tuesday 2 October 2012. The goal of this meeting is to bring together all groups that work on molecular and cellular biophysics and microscopy in the Netherlands. The meeting consists of plenary sessions, with invited presentations by (inter)national speakers, and special oral and poster sessions. Topics include the biophysics of living cells and their membranes, the cytoskeleton, molecular motors, DNA- and RNA-protein interactions, theoretical biophysics, systems biology and networks, photosynthesis, and new developments in light and electron microscopy, imaging and spectroscopy, force probes, (bio)chemical synthesis, and nanotechnology.